A role for the asexual spores in infection of Fraxinus excelsior by the ash-dieback fungus Hymenoscyphus fraxineus

This is the final version of the article. Available from Springer Nature via the DOI in this record.There is another ORE record for this article: http://hdl.handle.net/10871/33324The invasive pathogen, ash dieback fungus Hymenoscyphus fraxineus, is spreading rapidly across Europe. It shows high levels of outcrossing and limited population structure, even at the epidemic front. The anamorphic (asexual) form produces prolific conidia, thought to function solely as spermatia (male gametes), facilitating gene flow between sympatric strains. Here, we show that conidia are capable of germination on ash leaves and in vitro, and can infect seedlings via leaves or soil. In leaves, germlings form structures resembling fruiting bodies. Additionally, H. fraxineus colonises ash debris and grows in soil in the absence of ash tissues. We propose an amended life-cycle in which wind-dispersed, insectvectored or water-spread conidia infect ash and may sporulate in planta, as well as in forest debris. This amplifies inoculum levels of different strains in ash stands. In combination with their function as spermatia, conidia thus act to maximise gene flow between sympatric strains, including those originally present at low inoculum. Such mixing increases evolutionary potential, as well as enhancing the likelihood of gene introgression from closely-related strains or assimilation of further genetic diversity from parental Asian populations. This scenario increases the adaptability of H. fraxineus to new climates and, indeed, onto new host species.This work was funded by a grant from the BBSRC to the Nornex Consortium, BBS/E/J/000CA523, in association with DEFRA. We thank Dr Chris Thornton for useful discussions concerning fungal pathogens of soil and Dr Sreedhar Kilaru for kindly providing primers

A role for the asexual spores in infection of Fraxinus excelsior by the ash-dieback fungus Hymenoscyphus fraxineus

This is the final version of the article. Available from Springer Nature via the DOI in this record.There is another ORE record for this article: http://hdl.handle.net/10871/23613The invasive pathogen, ash dieback fungus Hymenoscyphus fraxineus, is spreading rapidly across Europe. It shows high levels of outcrossing and limited population structure, even at the epidemic front. The anamorphic (asexual) form produces prolific conidia, thought to function solely as spermatia (malegametes), facilitating gene flow between sympatric strains. Here, we show that conidia are capable of germination on ash leaves and in vitro, and can infect seedlings via leaves or soil. In leaves, germlings form structures resembling fruiting bodies. Additionally, H. fraxineus colonises ash debris and grows in soil in the absence of ash tissues. We propose an amended life-cycle in which wind-dispersed, insectvectored or water-spread conidia infect ash and may sporulate in planta, as well as in forest debris. This amplifies inoculum levels of different strains in ash stands. In combination with their function as spermatia, conidia thus act to maximise gene flow between sympatric strains, including those originally present at low inoculum. Such mixing increases evolutionary potential, as well as enhancing the likelihood of gene introgression from closely-related strains or assimilation of further genetic diversity from parental Asian populations. This scenario increases the adaptability of H. fraxineus to new climates and, indeed, onto new host species.This work was fund by a grant from the BBSRC to the Nornex Consortium, BBS/E/J/000CA523, in association with DEFRA

The Impact of Shape on the Perception of Euler Diagrams

Euler diagrams are often used for visualizing data collected into sets. However, there is a significant lack of guidance regarding graphical choices for Euler diagram layout. To address this deficiency, this paper asks the question `does the shape of a closed curve affect a user's comprehension of an Euler diagram?' By empirical study, we establish that curve shape does indeed impact on understandability. Our analysis of performance data indicates that circles perform best, followed by squares, with ellipses and rectangles jointly performing worst. We conclude that, where possible, circles should be used to draw effective Euler diagrams. Further, the ability to discriminate curves from zones and the symmetry of the curve shapes is argued to be important. We utilize perceptual theory to explain these results. As a consequence of this research, improved diagram layout decisions can be made for Euler diagrams whether they are manually or automatically drawn

Pilot Study Of Nutrient Loading In A Wet Detention Lake

Florida is surrounded by water, and its many internal lakes and rivers have long been recognized for their excellent fishing and boating. This notoriety draws land developers to the lake shores to establish residential and commercial infrastructure. This land development brings with it flood plain alteration, water level stabilization, and increased nutrients which cause adverse impacts to our lakes. In response, the United States Environmental Protection Agency (EPA) passed the Federal Clean Water Act (CWA) in 1972 which set the framework for the water quality standards for the entire United States. As a result of the CWA many point sources were eliminated, but in the process it became apparent that nonpoint source loads represented even more of a threat. To further study the physical and chemical characteristics of urban runoff the Nationwide Urban Runoff Program (NURP) was established in 1978. This research lead to a series of management options, named Best Management Practices (BMPs) which proposed various structural and non-structural methods to reduce nutrient loads. But the research and data collection on the effectiveness of these systems to remove nutrients is in its infancy. The main objective of this study was to generate accurate and effective water quality and water quantity data that future stormwater management decisions can be based upon. More specific, this study established automatic monitoring sites throughout the City of Kissimmee, Florida to determine the pollutant loadings into the tributaries of Lake Tohopekaliga. These monitoring sites are located such that inflows from outside the city limits can be isolated and external pollutant loads quantified. Also, additional internal monitoring sites were established to determine the pollutant loads of internal sections of the city. Data from these internal monitoring sites will also be used to determine the variable pollutant removal efficiencies and hydraulic fluctuations of natural, irregular riverine systems. The secondary objective of this study was to perform a pilot study using the discrete grab samples in tandem with the continuous hydraulic and hydrologic data from the monitoring stations. An existing lake within the project limits was chosen for the pilot study area. Monitoring stations are located at the influent and effluent sections of the lake which provided data on the hydraulic and hydrologic parameters. The pilot study determined the nutrient loads to and from the lake and checked for any seasonal variations in pollutant loading or removal efficiencies. For the purpose of this pilot study, only total nitrogen and total phosphorous were examined for two monitoring sites. The nutrient removal efficiency was performed using both the event mean concentration method and the summation of loads method to check for seasonal variation. There were no storm event concentrations available for used in this analysis, however, there were 25 discrete grab samples collected on a bi-monthly basis over a twelve month period. This data was used with corresponding five-minute rainfall and flow data from both the inflow and outflow points. The results of this study did not reveal any seasonal variation in the nutrient concentrations either flowing into or out from the lake. Although there were some relatively lower values in late spring, the concentration levels of total nitrogen did not seem to vary significantly from its mean value of 0.90 mg/l throughout the year. The concentration levels of total phosphorus did range from 0.02 mg/l to 0.48 mg/l, but not in relation to either season or flow volume fluctuations. The lake showed no net removals of total nitrogen and was actually found to be releasing total phosphorus to the downstream receiving waters. The findings of this study are limited due to the fact that the period of pilot study was only for twelve months and there were no rainfall events used in the analysis. Rainfall events are typically high sources of nutrient loads to a lake. The lower efficiencies were probably due to missing the actual higher nutrient load concentrations during the rainfall event. However, even considering the lack of event data, the nutrient removal efficiency for the pond was still low. This analysis did serve well as a basis for performing future analysis once additional data, including rainfall events, has been collected

Hyper-Raman scattering analysis of the vibrations in vitreous boron oxide

Hyper-Raman scattering has been measured on vitreous boron oxide, $v-$B$_2$O$_3$. This spectroscopy, complemented with Raman scattering and infrared absorption, reveals the full set of vibrations that can be observed with light. A mode analysis is performed based on the local D$_{3h}$ symmetry of BO$_3$ triangles and B$_3$O$_3$ boroxol rings. The results show that in $v-$B$_2$O$_3$ the main spectral components can be succesfully assigned using this relatively simple model. In particular, it can be shown that the hyper-Raman boson peak arises from external modes that correspond mainly to librational motions of rigid boroxol rings.Comment: 13 pages, 11 figures, 2 table

Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.

Even distribution of peroxisomes (POs) and lipid droplets (LDs) is critical to their role in lipid and reactive oxygen species homeostasis. How even distribution is achieved remains elusive, but diffusive motion and directed motility may play a role. Here we show that in the fungus Ustilago maydis ∼95% of POs and LDs undergo diffusive motions. These movements require ATP and involve bidirectional early endosome motility, indicating that microtubule-associated membrane trafficking enhances diffusion of organelles. When early endosome transport is abolished, POs and LDs drift slowly towards the growing cell end. This pole-ward drift is facilitated by anterograde delivery of secretory cargo to the cell tip by myosin-5. Modelling reveals that microtubule-based directed transport and active diffusion support distribution, mobility and mixing of POs. In mammalian COS-7 cells, microtubules and F-actin also counteract each other to distribute POs. This highlights the importance of opposing cytoskeletal forces in organelle positioning in eukaryotes.We thank Dr G. Dagdas, Dr S. Kilaru, Mr M. Schlick and Mrs T. Schrader for technical support. We thank Professor N.J. Talbot for helpful comments on the manuscript. This work was supported by the Biotechnology & Biological Sciences Research Council (BB/J009903/1 to G.S.). S.C.G. was supported by a fellowship from the Portuguese Foundation for Science and Technology (FCT) (SFRH/BD/73532/2010). J.M. is supported by a Wellcome Trust Institutional Strategic Support Award (WT097835MF)